This page is under construction. 
July 2, 2017
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DNA extraction

• Agencourt

​                Please find the protocol here.

• Squish prep 

​                This is for single fly, or anything equivalent. 

• QuickExtract

​                This is for single fly, or anything equivalent. 

• Qubit

PCR
    Primer design
    PCR (Taq/ OneTaq/ Phusion)
    PCR product purification (column/ beads)
​    Sanger sequencing

Library prepartion
    RNA extraction (Trizol)
    RNAseq library preparation (illumina)
    miSeq library preparation

Crispr
    gRNA/ ssODN design
    gRNA synthesis
    Fly embryo injection